There is only one spectral feature that clearly shows up on a Venus-like planet and not on an Earth-like one: Sulfur Dioxide (SO2). SO2 reacts with water vapor, so if it is present, it would rule out a wet Earth-like planet and confirm a dry Venus-like one

The researchers also estimated how much observing time would be required for JWST to see each of the different chemical signatures, and confirmed that it’s doable within a reasonable time frame.

The best signature for exo earth is to look for methane , there are absorption features of methane, that don’t show up on Venus , and can be clearly distinguished from co2 , further, if methane is accompanied by oxygen, it may be evidence of life

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